Figure 6From: Identification and characterization of a novel fumarase gene by metagenome expression cloning from marine microorganismsThe thermostability of recombinant FumF protein. The enzyme was pre-incubated at temperature ranging from 15 to 70°C at optimum pH. Subsequently, the residual activity was determined with fumarate as the substrate at 55°C in 100 mM glycine/NaOH buffer (pH 8.5).Back to article page