Skip to main content
Figure 3 | Microbial Cell Factories

Figure 3

From: Identification and characterization of a novel fumarase gene by metagenome expression cloning from marine microorganisms

Figure 3

SDS-PAGE of recombinant FumF protein. Proteins were separated by 12% (w/v) SDS-PAGE and then stained with Coomassie brilliant blue G-250. Lane 1, molecular weight standards; Lane 2, total protein of E. coli BL21(DE3)pLysS harboring empty pETBlue-2 (control); Lane 3, total protein of E. coli BL21(DE3)pLysS harboring the recombinant fumF in pETBlue-2 without induction by IPTG; Lane 4, total protein of E. coli BL21(DE3)pLysS harboring the recombinant fumF in pETBlue-2 induced by addition of 0.5 mM IPTG; Lane 5, sample purified by the Ni-NTA column method. The recombinant FumF protein is indicated by the black arrow.

Back to article page

Microbial Cell Factories

ISSN: 1475-2859

Contact us