Table 4 Description of plasmids used in development of the rTf expression system
From: High-level production of animal-free recombinant transferrin from Saccharomyces cerevisiae
Plasmid | Description |
|---|---|
pSAC35 | 2 μm-derived yeast episomal expression vector |
pDB2506 | pSAC35 with mHSA/MFα1-leader-rTf cassette at Not I site |
pDB2536 | pSAC35 with mHSA/MFα1-leader-rTf (N413Q, N611Q) cassette at Not I site |
pDB2690 | pSAC35 with PDI1 inserted at Xcm I site after REP2 |
pDB2711 | pDB2690 with mHSA/MFα1-leader-rTf (N413Q, N611Q) cassette at Not I site |
pDB2929 | pDB2690 with mHSA-leader-rTf (N413Q, N611Q) at Not I site |
pDB3557 | pDB2690 with invertase-leader-rTf (N413Q, N611Q) at Not I site |
pDB3237 | pDB2690 with invertase-leader-rTf (S415A, T613A) at Not I site |
pDB3768 | pDB2690 with invertase-leader-rTf (S32A, S415A, T613A) at Not I site |
pDB3235 | pSAC35 with invertase-leader-rTf (S415A, T613A) at Not I site |
pDB3236 | pSAC35 with invertase-leader-rTf (S415A, T613A) at Not I site |