Table 3 Yeast strains used in this work
From: High-level production of animal-free recombinant transferrin from Saccharomyces cerevisiae
Strain | Description/genotype | Reference |
|---|---|---|
JRY188 | cir0 MATa, leu2-3, leu2-112, ura3-52, trp1, his4, sir3, rme, GAL | [36] |
DB1 | cir0 MAT a, leu2-3, leu2-112 | [19] |
DS569 | rHA-overproducing strain derived from DB1 by chemical mutagenesis. Known genotype: cir0 MAT a, leu2-3, leu2-112 | |
DYB7 | rHA-overproducing strain derived from DS569 by chemical mutagenesis. Known genotype: cir0 MAT a, leu2-3, leu2-112 ubc4 ura3 yap3::URA3 lys2 hsp150::LYS2 | [37] |
DP9 | DYB7 with PDI1, URA3 and YIplac211 integrated at the PDI1 locus | This work |