Figure 7From: High-level production of animal-free recombinant transferrin from Saccharomyces cerevisiaeCharacterization of rTf. (A) Urea gel analysis of rTf (S415A, T613A). 10 μg samples of purified recombinant apo-transferrin (Lane 1) and holo-transferrin (Lane 2) derived from DYB7 [pDB3237] were separated on 6% TBE Urea PAGE (Invitrogen) and stained with Coomassie G250 (Pierce). (B) Deconvolved mass spectra from analysis of plasma-derived and rTf samples using ESI-TOF mass spectrometry. i) Plasma-derived transferrin (Calbiochem), ii) rTf (S415A, T613A) derived from DYB7 [pDB3237], iii) rTf (S32A, S415A, T613A) derived from DYB7 [pDB3768].Back to article page