Figure 4
From: High-level production of animal-free recombinant transferrin from Saccharomyces cerevisiae
Effects of alternative leaders on rTf N-terminus. 3 μl of five-day BMMD SFC supernatant was analyzed per lane by non-reducing SDS-PAGE (4-12% Bis-Tris NuPAGE®, MOPS buffer, Invitrogen). Track loadings were: Lane 1) SeeBlue® Plus2 protein standards (Invitrogen). Lane 2) DYB7 [pSAC35] negative control. Lane 3) DYB7 [pDB2711] with the modified HSA-pre and MFα1-pro leader. Lane 4) DYB7 [pDB2929] with the modified HSA-pre leader. Lane 5) DYB7 [pDB3557] with the S. cerevisiae invertase leader.