Figure 6
Fed-batch cultivations of E. coli K12 in minimal media initially supplemented with 5 g/L glucose. The scattered light and DCW are shown in black, the pH-value and the DOT in red and blue, respectively. The feed solution was composed of 500 g/L glucose and 70 g/L (NH4)2HPO4. All cultivations were carried out at a k L a-value of 460 1/h (A) linear feeding in microfluidic MTP with 2 mg/h (= 4 g/L/h with reference to 500 μL initial culture volume) (B) exponential feeding in microfluidic MTP with μ set = 0.2 1/h (C) exponential feeding in 2 L stirred tank reactor with μ set = 0.2 1/h