Table 3 Yeast strains and plasmids used in this study.
From: Improved vanillin production in baker's yeast through in silico design
Yeast Strain | Relevant genotype | Reference | ||
|---|---|---|---|---|
VAN286 | MAT a his3 D1 leu2 D0 met15 D0 ura3 D0 adh6::LEU2 bgl1::KanMX4 PTPI1::3DSD [AurC]::HsOMT [NatMX]::ACAR [HphMX] | Hansen et al. 2009 [5] | ||
VG0 | MAT a his3 D1 leu2 D0 met15 D0 ura3 D0 adh6::LEU2 bgl1::KanMX4 PTPI1::3DSD [AurC]::HsOMT [NatMX]::ACAR [HphMX]::UGT72E2 [HIS3] | This study | ||
VG1 | MAT a his3 D1 leu2 D0 met15 D0 ura3 D0 adh6::LEU2 bgl1::KanMX4 PTPI1::3DSD [AurC]::HsOMT [NatMX]::ACAR [HphMX]::UGT72E2 [HIS3] gdh1 | This study | ||
VG2 | MAT a his3 D1 leu2 D0 met15 D0 ura3 D0 adh6::LEU2 bgl1::KanMX4 PTPI1::3DSD [AurC]::HsOMT [NatMX]::ACAR [HphMX]::UGT72E2 [HIS3] pdc1 | This study | ||
VG3 | MAT a his3 D1 leu2 D0 met15 D0 ura3 D0 adh6::LEU2 bgl1::KanMX4 PTPI1::3DSD [AurC]::HsOMT [NatMX]::ACAR [HphMX]::UGT72E2 [HIS3] pdc1 gdh11 | This study | ||
VG4 | MAT a his3 D1 leu2 D0 met15 D0 ura3 D0 adh6::LEU2 bgl1::KanMX4 PTPI1::3DSD [AurC]::HsOMT [NatMX]::ACAR [HphMX]::UGT72E2 [HIS3] pdc1 gdh1 GDH2::PPGK1-GDH2 | This study | ||
Plasmid | Gene content | Plasmid type | Selection marker | Reference |
pJH589 | EndD (E. coli) | CEN-ARS (S. cerevisiae) | URA3 | Hansen et al. [5] |
pJH665 | UGT72E2 (Arabidopsis thaliana) | CEN-ARS (S. cerevisiae) | URA3 | Hansen et al. [5] |
pARB021 | UGT72E2 (Arabidopsis thaliana) | Integration (S. cerevisiae) | HIS3 | This work |
pWJ1042 | Recyclable URA3 (Kluyveromyces lactis) | CEN-ARS (S. cerevisiae) | Reid et al. [51] | |
pPGK1-GDH2 | PPGK1-GDH2 (S. cerevisiae) | Integration (S. cerevisiae) | KanMX3 | Nissen et al. [8] |