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Table 2 Physiological parameters for the reference and metabolically engineered strains in chemostat cultivation at dilution rate 0.1 h-1.

From: Improved vanillin production in baker's yeast through in silico design

Strain

VG0

VG2

VG4

Engineered Genotype

 

pdc1∆

pdc1 ∆gdh1∆GDH2

YS X (gDW.gglc-1)

0.151 ± 0.008

0.159 ± 0.003

0.115 ± 0.005

YS eth (geth.gglc-1)

0.283 ± 0.005

0.290 ± 0.008

0.32 ± 0.01

YS gly (mggly.gglc-1)

3.4 ± 0.3

2.1 ± 0.4

11 ± 9

YS acet (mgacet.gglc-1)

7.3 ± 0.3

7.6 ± 0.3

4 ± 2

YS PAC (mgPAC.gglc-1)

27 ± 2

23.5 ± 0.3

15.5 ± 0.3

YS PAL (mgPAL.gglc-1)

7 ± 2

9 ± 2

7.2 ± 0.2

YS VG (mgVG.gglc-1)

4 ± 1

5.6 ± 1

4.16 ± 0.07

rs(mmolglc.gDW-1.h-1)

3.9 ± 0.2

3.5 ± 0.3

4.8 ± 0.2

reth(mmoleth.gDW-1.h-1)

4.3 ± 0.3

3.9 ± 0.3

6.1 ± 0.4

rgly(mmolgly.gDW-1.h-1)

0.026 ± 0.003

0.014 ± 0.004

0.10 ± 0.08

racet(mmolacet.gDW-1.h-1)

0.086 ± 0.006

0.079 ± 0.006

0.05 ± 0.003

rPAC(mmnolPAC.gDW-1.h-1)

0.12 ± 0.01

0.10 ± 0.01

0.087 ± 0.03

rPAL(mmolPAL.gDW-1.h-1)

0.036 ± 0.008

0.040 ± 0.007

0.045 ± 0.003

rVG(mmolVG.gDW-1.h-1)

0.009 ± 0.003

0.011 ± 0.002

0.012 ± 0.001

  1. Substrate yields for biomass (X), ethanol (eth), glycerol (Gly), atetate (Ace), protocathechuic acid (PAC), protocathechuic adehyde (PAL) and vanillin β-D-glucoside (VG) on glucose (glc) are represented by YS X (or metabolite). Specific glucose uptake rate is represented by rS. Specific production rate for ethanol, glycerol, acetate, PAC, PAL and VG are represented by rmetabolite.
  2. Triplicates of all chemostats were performed.