Improved vanillin production in baker's yeast through in silico design

Table 1 Physiological parameters for the reference and metabolically engineered strains in batch cultivation.

Strains	Engineered Genotype	μ_max^a	Y_{S X}^b	Y_{S EtOh}^c	Y_{S gly}^d
VG0		0.14	0.10	0.23	0.05
VG1	gdh1∆	0.10	0.07	0.25	0.03
VG2	pdc1∆	0.20	0.14	0.23	0.07
VG3	pdc1 ∆gdh1∆	0.11	0.10	0.27	0.05
VG4	pdc1 ∆gdh1∆ ↑GDH2	0.17	0.17	0.25	0.07

^aMaximum specific growth rate, h^-1.
^bOverall yield of biomass on substrate, g_DW.g_glc^-1
^cOverall yield of ethanol on substrate, g_eth.g_glc^-1
^dOverall yield of glycerol on substrate, g_Gly.g_glc^-1
The biomass overall yield in glucose (Y_{S X}) was calculated based on all the biomass obtained after glucose and ethanol consumption. Ethanol and glycerol yields were calculated based on production only in the glucose consumption phase.

ISSN: 1475-2859