Figure 1
From: Improved vanillin production in baker's yeast through in silico design
Schematic representation of the de novo VG biosynthetic pathway in S. Cerevisisae(as designed by Hansen et al [5]). Metabolites are shown in black, enzymes are shown in black and in italic, cofactors and additional precursors are shown in red. Reactions catalyzed by heterologously introduced enzymes are shown in red. Reactions converting glucose to aromatic amino acids are represented by dashed black arrows. Metabolite secretion is represented by solid black arrows where relative thickness corresponds to relative extracellular accumulation. 3-DSH stands for 3-dedhydroshikimate, PAC stands for protocathechuic acid, PAL stands for protocatechuic aldehyde, SAM stands for S-adenosylmethionine. 3DSD stands for 3-dedhydroshikimate dehydratase, ACAR stands for aryl carboxylic acid reductase, PPTase stands for phosphopantetheine transferase, hsOMT stands for O-methyltransferase, and UGT stands for UDP-glycosyltransferase. Adapted from Hansen et al. [5].