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Table 2 Primers used in the study

From: Cloning and biochemical characterization of a novel lipolytic gene from activated sludge metagenome, and its gene product

Primer

Sequence 5'-3'

Description

HTFP061

GTACAACGACACCTAGAC

Sequencing primer for pCC1FOS™

HTRP062

CAGGAAACAGCCTAGGAA

Sequencing primer for pCC1FOS™

M13 primer RV

CAGGAAACAGCTATGAC

Sequencing primer for pUC18

M13 primer M2

AGCTGTTCACCGAAGTGCTG

Sequencing primer for pUC18

EstMY -W1F

CGCCCCTTTCGACCAGCAACG

Genomic walking primer for estMY gene

EstMY -W2F

CTACGCCGACCTCACCGGCCT

Genomic walking primer for estMY gene

EstMY -W1R

GAGGGGTGTGCGGGGATGCG

Genomic walking primer for estMY gene

EstMY -W2R

GACGTAGCCGCCGCCGTGAAG

Genomic walking primer for estMY gene

EstMY -F

GGCAT ATG GCCGCGCCCGTTCCGCCCATCAG Nde I

Forward primer for estMY gene

EstMY -R

GGAAGCTT CTACGCTGCCGCCCTAGCGCCGATGHin dIII

Reverse primer for estMY gene

  1. The Nde I and Hin dIII sites are underlined. The start codon is in bold.