Table 1 Starting bacterial strains and plasmids used in this study

E. coli TOP10

lac х74 recA1 deoR F - mcrA ∆ (mrr-hsdRMS-mcrBC) ϕ80 lacZ∆M15∆ araD139∆ (ara-leu)7697 galU galK

Transgen

E. coli EPI300™-T1R

[F- e14-(McrA-) D(mcrC-mrr) (TetR) hsdR514 supE44 supF58 lacY1 or D(lacIZY)6 galK2 galT22 metB1 trpR55 l-]

Epicentre

E. coli BL21(DE3)

F-, ompT, hsdSB (rB-, mB-), dcm, gal, λ(DE3), pLysS, Cmr

Novogen

E. coli EPI300-FosD11L2

Positive clone from Fosmid genomic library, which carries the lipolytic gene

This study

E. coli TOP10-EstMY

Positive clone from sublibrary, which carries the EstMY gene fragment

This study

E. coli BL21(DE3)-EstMY

Positive clone, which carries the pEstMY-His expression vector

This study

Plasmids

pCC1FOS

Cloning vector; Chl^r

Epicentre

pUC18

Cloning vector; Ap^r

Takara

pET28a

Expression vector; Km^r

Novagen

FosD11L2

pCC1FOS, which carries the estMY gene cluster (31 kb)

This study

pUC18-EstMY

pUC18, which carries the complete lipolytic gene (estMY)

This study

pEstMY-His

pET28a carrying amplified Hin dIII -Nde I fragment containing lipolytic gene (estMY)

This study