Figure 6From: Cloning and biochemical characterization of a novel lipolytic gene from activated sludge metagenome, and its gene productApparent temperature optimum of esterase EstMY. Relative activity of p-NP-caprylate hydrolysis at different temperatures by purified EstMY. The activity was determined at different temperatures at pH 8.0 in 50 mM Tris-HCl buffer. The activity at 35°C was set as 100% (3,936 U/ml). All measurements were performed in triplicate.Back to article page