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Table 1 Intracellular glycerol contents (mM).

From: Saccharomyces cerevisiae glycerol/H+ symporter Stl1p is essential for cold/near-freeze and freeze stress adaptation. A simple recipe with high biotechnological potential is given

 

Experimental procedures

Cell type

YPGE, 20 h 4°C

YPGE, 30°C

YPD, 20 h 4°C

YPD, 30°C

Wt

453.8 ± 0.011

153.0 ± 0.021

1.190 ± 0.016

0.750 ± 0.35∂

stl1Δ

0.196 ± 0.019

0.167 ± 0.014

1.205 ± 0.011

1.091 ± 0.012

gpd1Δgpd2Δ

427.1 ± 0.020

138.8 ± 0.023

0.981 ± 0.013

1.1 ± 0.021∂

  1. ∂ Results on YPD at 30°C were obtained before (Oliveira and Lucas, 2004) and yielded residual amounts on wt and gpd1Δgpd2Δ..
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Microbial Cell Factories

ISSN: 1475-2859

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