Figure 3From: Saccharomyces cerevisiae glycerol/H+ symporter Stl1p is essential for cold/near-freeze and freeze stress adaptation. A simple recipe with high biotechnological potential is givenInduction of active glycerol transport. (A) Cells of wt and gpd1Δgpd2Δ mutant were grown to exponential phase on YPGE at 30°C (stripped bars) and subjected to incubation during 20 h at 4°C (full bars). (B) The same batches of cells were used to detect the levels of Stl1p expression by Western Blot using STL1-ZZ construction: 1 and 4 - gpd1Δgpd2Δ cells at 30°C and 4°C respectively; 2 and 3 - wt cells at 30°C and 4°C respectively.Back to article page