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Figure 1 | Microbial Cell Factories

Figure 1

From: Quantification of the physiochemical constraints on the export of spider silk proteins by Salmonella type III secretion

Figure 1

An overview of the system is shown. A. The protein to be secreted is controlled by a genetic AND gate that is responsive to a genetic circuit that responds to psicA inducer added to the culture media (IPTG). The needle image is from reference [48]. B. A map of the pCASP plasmid is shown. The black features represent transcriptional terminators. C. Three promoters used in this study are shown with critical features marked. The red box represents the invF:sicA operator binding site [14]. The blue boxes represent the LacO1 operator-binding site. Various spacings of Lac operators are generated and tested here to find optimal separation for LacO looping by the LacI tetramer (green circles).

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Microbial Cell Factories

ISSN: 1475-2859

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