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Table 3 Media pricing

From: Expression and purification of ELP-intein-tagged target proteins in high cell density E. coli fermentation

Component

NBS1

TB*1

Feed 11

Feed 21

Feed 31

Feed 41

Feed 51

NBS fed batch (Feed 5)1

NBS (bulk)2

TB* (bulk)

Feed 5 (bulk)

NBS fed batch (Feed 5, bulk)

NBS fed batch (Feed 5, commodity)4

Ammonium phosphate

2.12

      

1.41

0.46

  

0.31

 

Ammonium chloride

  

0.31

1.25

 

2.5

1.25

0.42

  

1.09

0.36

 

Ammonium sulfate

   

1.47

 

6.81

1.47

0.49

  

1.28

0.43

 

Boric acid

0.006

      

0.004

0.006

  

0.004

 

Corn steep liquor

            

.001

Corn syrup

            

0.07

Cupric sulfate

0.0006

      

0.0004

0.0006

  

0.0004

 

Ferric chloride

0.003

      

0.002

0.003

  

0.002

 

Glucose

3.65

 

94.89

94.89

94.89

94.89

94.89

34.03

0.7

 

18.2

6.53

 

Glycerol

 

0.76

       

0.14

   

Magnesium sulfate

0.59

 

0.89

0.89

0.89

0.89

0.89

0.69

0.02

 

0.35

0.13

0.13

Manganese chloride

0.008

      

0.005

0.008

  

0.005

 

Monosodium glutamate

    

17.32

        

Potassium phosphate dibasic

0.5

2.07

     

0.33

0.18

0.76

 

0.12

0.12

Potassium phosphate monobasic

0.22

0.25

     

0.15

0.15

0.18

 

0.10

0.10

Proline

 

6.91

       

6.913

   

Sodium chloride

0.002

      

0.001

0.002

  

0.001

 

Sodium molybdate

0.002

      

0.001

0.002

  

0.001

 

Sulfuric acid

0.002

      

0.001

0.002

  

0.001

 

Thiamine

0.0003

      

0.0002

0.0003

  

0.0002

 

Tryptone

 

1.85

       

1.853

   

Yeast extract

0.96

4.63

    

0.96

0.96

0.963

4.633

0.963

0.96

 

Zinc sulfate

0.0006

      

0.0004

0.0006

  

0.0004

 

Cost per L (US Dollars)

8.06

16.47

96.09

98.50

113.10

105.09

99.46

38.50

2.49

14.47

21.88

8.95

0.42

  1. 1Prices based on 500 g reagent grade chemicals from Fisher Scientific.
  2. 2Bulk scale prices based on 50 kg reagent grade chemicals from Fisher Scientific, when available. Otherwise, price based on largest reagent grade size available (1-10 kg). Bulk scale pricing was not taken into account for trace materials.
  3. 3Bulk discounts not available.
  4. 4Commodity scale replaces (mass for mass) glucose with corn syrup, nitrogen sources with corn steep liquor, and eliminates trace metals.