Table 2 Small-scale purification of β-gal and OPH-S5 based on two rounds of inverse transition cycling.
A. β-galactosidase |  |  |  |
|---|---|---|---|
Step | Specific activity (U/mg) | Purification fold | Yield (mg/L) |
Clarified lysate | 5096 | 1 | Â |
Soluble contaminants | 466 | Â | Â |
Purified precursor, before cleaving | 76656 | 15 | Â |
Purified product | 135017 | 25.5 | 177 |
B. OPH-S5 | Â | Â | Â |
Step | Specific activity (U/mg) | Purification fold | Yield (mg/L) |
Clarified lysate | 11 | 1 | Â |
Soluble contaminants | 1.2 | Â | Â |
Purified precursor, before cleaving | 751 | 68 | Â |
Purified product | 1186 | 108 | 83 |