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Figure 1 | Microbial Cell Factories

Figure 1

From: Expression and purification of ELP-intein-tagged target proteins in high cell density E. coli fermentation

Figure 1

IPTG induction of ELP-tagged targets in TB shake flasks. Comparison of IPTG-induced expression (denoted by *) versus leaky expression in TB medium using the BLR (DE3) strain. In each case the overexpressed precursor protein is marked. 1: α-hemoglobin stabilizing protein (EI-AHSP = 73 kDa), 2: β-lactamase (EI-β-lac = 90 kDa), 3: β-galacotosidase (EI-β-gal = 177 kDa), 4: green fluorescent protein (EI-GFP = 88 kDa), 5: glutathione S-transferase (EI-GST = 87 kDa), and 6: maltose binding protein (EI-MBP = 102 kDa).

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