Display of wasp venom allergens on the cell surface of Saccharomyces cerevisiae

Table 2 List of primers

Primer name	Sequence	Application
vesv1_fw	ATGGAAGAAAATATGAATTTAAAG	Cloning genes from RNA
vesv1_rv	TTAAATTATCTTCCCCTTGTTATTG
vesv2.0101_fw	TCCGAGAGACCGAAAAGAG
vesv2.0101_rv	TTAGTTGACGGCTTCTGTCAC
vesv2.0201_fw	GACAGAACAATTTGGCCTAAG
vesv2.0201_rv	CTAAAAGTTTAACGGTGTGTTTTC
vesv5_fw	ATGGAAATTAGTGGGCTC
vesv5_rv	TTACTTTGTTTGATAAAGTTCCTC
28SrRNAvesv_fw	AGCGTCAGCGGCGCTG
28SrRNAvesv_rv	GAGACACTGACCGCGCTTG
SD_vesv1_fw	NheI ATCAGCTAGC GGACCCAAATGTCCTTTTAATTC	Cloning genes into surface display vector
SD_vesv1_rv	BamHI CGATGGATCC AATTATCTTCCCCTTGTTATTGC
SD_vesv2_fw	NheI ATCAGCTAGC TCCGAGAGACCGAAAAG
SD_vesv2_rv	BamHI CGATGGATCC GTTGACGGCTTCTGTC
SD_vesv5_fw	NheI ATCAGCTAGC AACAATTATTGTAAAATAAAATG
SD_vesv5_rv	BamHI CGATGGATCC CTTTGTTTGATAAAGTTC
zeo_fw	KpnI TAGATTGGTACC CCCACACACCATAGCTTC	Cloning zeocin resistance gene
zeo_rv	KpnI GTCCTCGGTACC AGCTTGCAAATTAAAGC

The first group of primers was used for amplification of four allergen genes and control 18 S rRNA from wasp venom sac RNA. The second group of primers was used for generating fragments with NheI and BamHI overhangs for cloning into surface display vector. The last set of primers was used for cloning zeocin resistance cassette with KpnI overhangs. The restriction sites are underlined.

ISSN: 1475-2859