Figure 2

Vector map of a surface display vector with zeocin resistance. The original vector pCTCON2 [36] was modified by addition of zeocin resistance cassette, containing yeast TEF1 promoter, prokaryotic EM7 promoter, zeocin resistance gene ble from Streptoalloteichus hindustanus and transcription terminator CYC1. The allergen genes were cloned flush downstream the flexible peptide linker (Gly₄Ser)₃, which was connected to aga2 gene under control of galactose-inducible promoter GAL1. The allergen genes were also cloned in-frame with C-terminal myc tag. The vector additionally contained ampicillin resistance marker Amp(R) for selection in E. coli, tryptophan selection marker TRP1 for selection in S. cerevisiae, pUC origin of replication for E. coli, and CEN6/ARSH4 origin for vector replication in S. cerevisiae.