Figure 1
From: Display of wasp venom allergens on the cell surface of Saccharomyces cerevisiae
The principle of surface display using a-agglutinin system. The allergen of interest (here shown ves v 1) is cloned in-frame with aga2 gene and introduced into a yeast strain that can overexpress aga1 gene. The resulting proteins, Aga1 and Aga2-allergen fusion, get connected by disulfide bonds in the secretion process and are exported together on the surface of the cell where Aga1 remains attached to the cell wall by the glycosylphosphatidylinositol (GPI) anchor.