Single cell oils of the cold-adapted oleaginous yeast Rhodotorula glacialis DBVPG 4785

Table 3 Effects of glucose concentration on growth and lipid production of R. glacialis DBVPG 4785

Glucose (g L^-1)	μ (h^-1)	Y_L/S (g g^-1)	Y_L/X (g g^-1)	Y_X/S (g g^-1)	Q_X (g L^-1 h^-1)	Q_L (g L^-1 h^-1)	Cells (ml^-1)	Biomass (g L^-1)	Carbohydrates (g L^-1)	Lipids (g L^-1)
1.6	0.070^a	0.07^d	0.06^e	1.31^a	0.045^e	0.0030^f	1.2e+08^c	2.1^h	0.1^f	0.12^g
4	0.071^a	0.05^d	0.05^e	0.94^b	0.052^d	0.0026^f	3.5e+08^b	3.8^g	0.3^e	0.19^g
8	0.070^a	0.05^d	0.09^e	0.61^c	0.052^d	0.0044^e	4.0e+08^ab	4.9^f	0.8^d	0.42^f
16	0.072^a	0.09^c	0.20^d	0.44^d	0.056^c	0.011^d	4.2e+08^a	7.0^e	1.6^c	1.36^e
40	0.072^a	0.12^b	0.34^c	0.35^e	0.075^b	0.025^c	4.5e+08^a	14^d	3.7^b	4.74^d
80	0.065^b	0.15^a	0.55^b	0.28^f	0.076^b	0.042^b	4.4e+08^a	22^c	5.4^a	12^c
120	0.060^bc	0.16^a	0.68^ba	0.23^g	0.079^a	0.054^a	4.3e+08^a	28^b	4.8^a	19^b
160	0.057^c	0.16^a	0.68^ba	0.23^g	0.080^a	0.054^a	4.4e+08^a	33^a	5.0^a	22^a

The strain was cultured at 10°C in GMY medium containing different glucose concentration, as described in Methods section. The final concentration of biomass, cell-associated carbohydrates, and lipids, the specific growth rate (μ), the biomass/glucose, lipid/glucose, and lipid/biomass yield coefficients (Y_X/S, Y_L/S, and Y_L/X, respectively), and the volumetric productivities of biomass and lipids (Q_X and Q_L, respectively) are reported as a function of initial glucose concentration. The values are means of three independent experiments. Within a column, superscripts indicate statistically similar means, P > 0.05, n = 3.

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