Isolation of cell-free bacterial inclusion bodies

Table 1 Bacterial lysis methods for IB purification.

Lysis method		IB protein	DNase	Detergents	Tested viability	Reference
NON MECHANICAL LYSIS METHODS	LYSOZYME	LACVP1	Yes	Yes	No	[19–22]
		VP1LAC	Yes	Yes	No	[7–9, 19–27]
		V2LAC	Yes	Yes	No	[22]
		TSP	Yes	Yes	No	[23]
		VP1GFP	Yes	Yes	No	[7, 28–30]
		hDHFR	Yes	Yes	No	[7]
		Aβ42-BFP	Yes	Yes	No	[7]
		β-lactamase	Yes	Yes	No	[31]
		Prochymosin	No	Yes	No	[32]
		HET-s fungal prion	Yes	Yes	No	[33]
		Aβ42-GFP	Yes	Yes	No	[30, 34]
		Aβ42-BFP	Yes	Yes	No	[30]
		MalE-Bla and MalE31-Bla	No	No	No	[35]
		MalE-PhoA and MalE31-PhoA	No	No	No	[35]
	NON-IONIC DETERGENTS	CBDclosN-SAA	No	Yes	No	[36]
		SAA-6HisC	No	Yes	No	[36]
		Maltodextrin phosphorylase	No	Yes	No	[37]
		CBD_closSabA	No	Yes	No	[38]
MECHANICAL LYSIS METHODS	HOMOGENIZER	rhBMP-2	No	Yes	No	[17, 39]
		G-CSF	No	No	No	[40]
		rhMCSF	No	No	No	[41]
		rHEWL	No	Yes	No	[42]
		GFP	No	No	No	[43]
	FRENCH PRESS	EGD	No	No	No	[44]
		TvDAO	No	Yes	No	[45, 46]
		NS3 protein	No	Yes	No	[47]
	SONICATION	IFN-α	No	No	No	[48]
		β-galactosidase	No	No	No	[49]
		pGH	No	No	No	[50]
		Pre-β-lactamase	No	No	No	[51]
		Procathepsin B	No	Yes	No	[52]
COMBINED LYSIS METHODS	SONICATION + HOMOGENIZER	N^pro fusion proteins	No	Yes	No	[53]
	SONICATION + LYSOZYME or LYSOZYME + SONICATION	Prochymosin	No	Yes	Yes	[54]
		Cro-β-gal	Yes	Yes	Yes	[55]
		His-GST-GFP	Yes	No	No	[56]
		Prochymosin B	No	Yes	No	[54]
		CLIPB14 Serine protease	Yes	Yes	No	[57]
	FRENCH PRESS + LYSOZYME or LYSOZYME + FRENCH PRESS	PHA synthase	Yes	Yes	No	[58]
		Class II PHA synthase	Yes	Yes	No	[58]

ISSN: 1475-2859