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Table 5 Solvent fractionation to separate SCL/MCL-PHAs from SCL-PHAs.

From: Production of functionalized polyhydroxyalkanoates by genetically modified Methylobacterium extorquens strains

  

Monomeric composition of PHA1

PHA sample

Portion2[%]

C4:0

[mol%]

C5:0

[mol%]

C6:5

[mol%]

C6:0

[mol%]

C8:7

[mol%]

C8:0

[mol%]

Blend3

-

91.36 +/- 1.18

0.83 +/- 0.02

3.26 +/- 1.00

1.31 +/- 0.05

3.24 +/- 0.11

tr4

Acetone(-)5

90.84

96.55

0.48

1.47

0.47

1.03

-

Acetone(+)6

4.63

55.85 +/- 6.43

3.33 +/- 0.70

21.72 +/- 7.81

6.98 +/- 1.56

12.12 +/- 3.48

tr

Loss7

4.52

-

-

-

-

-

-

  1. 1CX:Y = double bond at position Y of monomeric chain length X;
  2. 2Portion after Soxhlet fractionation;
  3. 3Chloroform extract from biomass (n = 2);
  4. 4tr = detected in trace quantity;
  5. 5Acetone-insoluble fraction (n = 1);
  6. 6Acetone-soluble fraction (n = 3);
  7. 7PHA loss during Soxhlet extraction.