Skip to main content

Table 2 1D and 2D NMR analysis of selected PHA samples1.

From: Production of functionalized polyhydroxyalkanoates by genetically modified Methylobacterium extorquens strains

Co-substrate

1D Analysis

2D Analysis

C6=2

3HB as major peak (> 90%), 3HHx=3

Not performed.

C8=4

3HB as major peak (> 96%)

At least 5 minor components: 3HP, 3HHx, 3HHx=, 3HO and 3HO=.

C11=5

                                    Poor quality spectra.

-

3HB, 3HP (very minor)

No detectable unsaturated bonds

  1. 1These samples were chosen as representatives due to availability of enough material and equipment;
  2. 2Three (3) similar samples were analyzed;
  3. 3Since C6= was the co-substrate, it was concluded that the detected double bonds belonged to 3HHx=;
  4. 4Three (3) similar samples were analyzed;
  5. 5Two (2) similar samples were analyzed.