Figure 6

Overlay of the 2D spectra for a PHA sample derived from methanol and 7-octenoic acid metabolism. The 2D ¹H-¹³C HSQC, HMBC and H2BC spectra are shown as black, red and blue contour plots, respectively. Single and multiple bond correlations for HP (3HP), HH (3HHx), HH= (3HHx=), HO (3HO) and HO= (3HO=) are indicated according to the following nomenclature. Multiple bond (HMBC) and 2-bond (H2BC) correlation cross peaks are identified by two numbers: the first corresponding to the ¹H atom and the second relating to the ¹³C atom, e.g., HH6,4 describes the cross peak between H-6 and C-4 of 3-hydroxyhexanoate. Single correlation (HSQC) cross peaks are denoted by a single number (e.g., HP5, corresponds to the correlation of H-5 and C-5 for 3-hydroxypentanoate). Detailed analysis are provided as Appendix.