Figure 5

Secretion levels of yeast protease, lipase and rhIL-1 β in Z. bailii and ZbΔgas 1. (a) Secretion levels of protease in Z. bailii and Zb Δgas 1 grown in min media with 5% glucose. Open circles and open squares indicate wild type and Δgas 1 mutant transformed with control plasmid. Solid circles and solid squares indicate wild type and Δgas 1 mutant transformed with plasmid with XPR2 gene. (b) Secretion levels of lipase in Z. bailii and Zb Δgas 1 grown in min media with 5% glucose. Open circles and open squares indicate wild type and Δgas 1 mutant transformed with control plasmid. Solid circles and solid squares indicate wild type and Δgas 1 mutant transformed with plasmid with LIP1 gene. (c) Western blots for rhIL-1β of protein samples from supernatants and cell extracts of Z. bailii and Zb Δgas 1 transformants grown in YPD media with 5% glucose, inoculated at OD660 = 0,1. From left to right: (A) rhIL-1β; (B) negative control Z. bailii[pZ₅]; (C) (D) (E) (I) (L) (M) Z. bailii [pZ₅Kl rhIL-1β]; (F) (G) (H) (N) (O) (P) Zb Δgas 1 [pZ₅Kl rhIL-1β]. Time and sample types are indicated in the picture.