Figure 3

Morphology of Z. bailii and S. cerevisiae wild type and gas1 null mutant strains and their sensitivity to Calcofluor White and SDS. Cellular morphology: From A to H: dicroic and fluorescence microscope images of Z. bailii and S. cerevisiae wild type and gas 1 null mutant strains. Cells were grown in minimal medium and harvested in stationary phase of growth. Arrows indicate granules and enlarged vacuoles in mutant cells (panels C and G). Calcofluor White and SDS sensitivity of S. cerevisiae (BY4741) and Z. bailii (Zygo) gas 1 null mutants: 4 μl of cultures of the different strains (BY4741, BY4741 Δgas 1, Zygo and Zygo Δgas 1) were spotted starting with OD (660 nm) = 1 and then in 3 serial solution 1:10 (2,3,4) on rich solid medium (YPD) and on the same medium added with 12.5 μg ml^-1 of CFW or 50 μg ml^-1 SDS. Pictures were captured after 5 days of incubation at 30°C.