Figure 2From: Cloning of the Zygosaccharomyces bailii GAS 1 homologue and effect of cell wall engineering on protein secretory phenotypeDeletion of ZbGAS 1. (a) Schematic representation of the ZbGAS 1 deletion cassettes. Restriction enzymes used to obtain the constructs and PCR primers used to verify recombination are indicated, light grey bars represents ZbGAS 1 sequence. (b) Control PCR performed to verify the deletion of both the allelic copies of ZbGAS 1. Two independent mutant clones (A and B) were tested. In both clones it is possible to see the presence of bands corresponding to the two deletion cassettes and the absence of the band corresponding to the wild type gene copy.Back to article page