Skip to main content
Figure 2 | Microbial Cell Factories

Figure 2

From: Engineering the cell surface display of cohesins for assembly of cellulosome-inspired enzyme complexes on Lactococcus lactis

Figure 2

Growth profiles of L. lactis expressing CipA frags alone or as fusions with M6 cwa and/or NucA. Panels A, D and G represent cultures not induced with nisin, panels B, E, H represent cultures induced with 10 ng/mL nisin at inoculation (t = 0 hrs), and panels C, F, I represent cultures induced with 10 ng/mL nisin in log phase corresponding to an OD600 ≈ 0.3 (t = 4 hrs). Constructs were grouped according to their modular nature. Top panels depict constructs containing a single cohesin; Middle panels depict constructs containing two CipA modules; Lower panels depict constructs containing no cohesin modules. Black shapes indicate scaffolds containing a fusion with NucA, and white shapes indicate scaffolds where NucA has been removed. Solid lines represent scaffolds expressed with a cell wall anchor, and dotted lines represent scaffolds lacking the cell wall anchor. Experiments were repeated three times yielding identical trends between growth profiles.

Back to article page