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Table 4 Summary of productivity of galactose oxidase in different hosts

From: A comparative summary of expression systems for the recombinant production of galactose oxidase

straina

specific activity

[U·mg-1]

volumetric productivity

[U·L-1·h-1]

specific productivity

[mg product·g-1biomass·h-1]

E.coli ENI

180 ± 7 (n = 2)b

180 ± 7

0.15 ± 0.1

P. pastoris FxIc

10 (n = 1)

120

n.d.d

P. pastoris FxEc

260 ± 14 (n = 3)

610 ± 30

0.12 ± 0.09

P. pastoris PCE

250 ± 12 (n = 3)

580 ± 25

0.19 ± 0.1

  1. a See Table 1
  2. b n, number of replicates
  3. c GalOx was not purified from crude extract
  4. d n.d. not determined