Figure 5
From: A comparative summary of expression systems for the recombinant production of galactose oxidase
Specific activity of wtGalOx from F. graminearum expressed extracellularly in P. pastoris at 25°C for 216 h . The cultivation medium BMMY was either not supplemented with additional CuSO4 or cells were cultivated in the presence of either 0.5 mM or 1.0 mM CuSO4. After 216 h of cultivation the specific activity of GalOx in the supernatants was determined (dark grey bars). Additional 0.5 mM CuSO4 were added and the samples were incubated at 4°C and stirring overnight. After removal of excessive CuSO4 by gel filtration, the specific activity was determined again (light grey bars). Error bars represent standard deviation for 3 biological replicates and 2 assay replicates each.