Skip to main content

Table 2 Production of AppA in the cytoplasm of E. coli

From: Disruption of reducing pathways is not essential for efficient disulfide bond formation in the cytoplasm of E. coli

Sample

Δ Absorbance

Final OD of culture

Relative yield (%)

AppA in BL21 (DE3)

0.003 ± 0.004

2.60 ± 0.18

1.3 ± 1.3

AppA + DsbC in BL21 (DE3)

-0.003 ± 0.004

2.02 ± 0.07

-0.7 ± 1.2

AppA + Erv1p in BL21 (DE3)

0.039 ± 0.009

1.10 ± 0.02

6.2 ± 1.4

AppA + Erv1p + DsbC in BL21 (DE3)

0.420 ± 0.062

1.65 ± 0.01

100 ± 14.2

AppA in rosetta-gami

0.105 ± 0.020

1.91 ± 0.14

28.6 ± 3.0

AppA + DsbC in rosetta-gami

0.123 ± 0.007

2.03 ± 0.01

36.2 ± 1.9

  1. Data for the production of active E. coli phytase (AppA) grown in LB media at 30°C, where Δ absorbance is the change in absorbance over 20 minutes at 410 nm upon assaying the lysate (n = 4). Note that both the BL21 (DE3) strain and the rosetta-gami (Δgor ΔtrxB) strain contain pLysSRARE. As can be seen from the data the growth rate of both the BL21(DE3) pLysSRARE and rosetta-gami strains decreases upon co-expression of Erv1p resulting in a lower final OD600 of the culture after 4 hours of induction. Despite this the relative yield of active protein is much higher upon co-expression.