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Table 1 Production of PhoA in the cytoplasm of E. coli

From: Disruption of reducing pathways is not essential for efficient disulfide bond formation in the cytoplasm of E. coli

Sample

Δ Absorbance (mAU/min)

Final OD of culture

Relative yield (%)

PhoA in BL21 (DE3)

-0.068 ± 0.053

2.67 ± 0.20

-0.3 ± 0.3

PhoA + Erv1p in BL21 (DE3)

27.6 ± 0.6

1.73 ± 0.01

100 ± 2.1

PhoA in rosetta-gami

16.2 ± 0.6

1.59 ± 0.05

53.9 ± 3.6

PhoA + Erv1p in rosetta-gami

11.0 ± 0.8

1.27 ± 0.03

29.3 ± 1.8

  1. Data for the production of active E. coli alkaline phosphatase (PhoA) grown in LB media in shake flasks at 30°C, where Δ absorbance is the rate of change in absorbance per minute at 410 nm upon assaying the lysate (n = 4). Note that both the BL21 strain and the rosetta-gami (Δgor ΔtrxB) strain contain pLysSRARE. As can be seen from the data the growth rate of both the BL21(DE3) pLysSRARE and rosetta-gami strains decreases upon co-expression of Erv1p resulting in a lower final OD600 of the culture after 4 hours of induction. Despite this the relative yield of active protein is much higher upon co-expression.
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Microbial Cell Factories

ISSN: 1475-2859

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