Figure 6

Agglomerates of G-CSF IBs and cell debris after isolation. IBs often form agglomerates together with the remains of the bacterial cells after enzymatic lysis (A). Similar agglomerates can also be observed after homogenization (B) and sonication (C) when pH of the buffer, where the cells are resuspended, is very low (pH 2-4). However, after enzymatic lysis more amorphous matter can be observed between the IBs in the agglomerate compared to IBs after mechanical isolation methods. This amorphous material is probably bacterial cell debris together with the enzyme lysozyme.