Figure 4
From: Isolation of biologically active nanomaterial (inclusion bodies) from bacterial cells
SDS-PAGE analysis of protein (GFP) distribution between soluble and insoluble cell fraction after bacterial cell disruption. Target protein (GFP) was overexpressed in the form of ncIBs inside bacterial cells. Three mechanisms of bacterial cell disruption were studied: high pressure homogenization (H), sonication (S) and enzymatic lysis (L). After bacterial cell disruption soluble cell fraction (sn) and insoluble cell fraction (p) were separated with centrifugation and both samples were analysed with SDS-PAGE. Whole bacterial cells were also analysed (total cell proteins - Tcp). After IBs isolation from the cells, they were solubilised in N-lauroul sarcosine (presented as sIBs). The insoluble residue (r) that remains after solubilisation of IBs is also presented. The most abundant protein in the samples lysed with enzymatic lysis is enzyme Lysozyme. 1 - total cell proteins. 2 - Hsn. 3 - Hp. 4 - HsIBs. 5 - Hr. 6 - Ssn. 7 - Sp. 8 - SsIBs. 9 - Sr. 10 - Lsn. 11 - Lp. 12- LsIBs. 13 - Lr.