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Figure 10 | Microbial Cell Factories

Figure 10

From: Isolation of biologically active nanomaterial (inclusion bodies) from bacterial cells

Figure 10

Protein loss from IBs during sonication. NcIBs were isolated from bacterial cells using homogenization. They were thoroughly washed and resuspended in pure water. Isolated ncIBs were then treated as bacterial cells during sonication. Aliquots were taken from the samples every minute. GFP and G-CSF ncIBS were studied. Proteins are lost from ncIBs during sonication. The quantity of protein loss during sonication is almost four times higher compared to high-pressure homogenization. As during homogenization also at sonication more proteins are lost from IBs composed from G-CSF.

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