Table 5 Summary of plasmids used
From: Antibiotic-free selection in E. coli: new considerations for optimal design and improved production
Plasmid name | Derived from plasmid | Recombinant protein expressed | F1 origin | cer fragment | Kanamycin resistance gene | ccdA gene |
|---|---|---|---|---|---|---|
pET28c | - | + | - | + | - | |
pM1800 | pET28c | - | - | + | + | - |
pMH.P3.1 | pET28c | AlpA | + | - | + | - |
pM1816 | pM1800 | rEPA | - | + | + | - |
pSP301 | pM1800 | - | - | + | Rcb | + |
pSP1 | pM1816 | rEPA | - | + | - | + |
pSP2 | pSP301 | - | - | - | Rcb | + |
pSP3 | pSP2 | AlpA | - | - | Rcb | + |
pSP4 | pSP2 | rEPA | - | - | Rcb | + |
pSP5 | pSP301 | AlpA | - | + | Rcb | + |
pSP6 | pSP301 | rEPA | - | + | Rcb | + |