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Table 4 Scale-up of antibiotic-free system

From: Antibiotic-free selection in E. coli: new considerations for optimal design and improved production

Fermenter scale

1 L

30 L

Induction time

3 h

20 h

3 h

20 h

A600

72

47

73

55

P+ %

100

98

100

100

rEPA (mg/L)

337

603

194

275

  1. No marked differences appear between 1 liter and 30 liters fermentation for the antibiotic-free system in terms of both, cell density and % of P+ cells.
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Microbial Cell Factories

ISSN: 1475-2859

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