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Table 2 Example of Helicobacter pylor i Urease produced in erlen flask in absence of kanamycin

From: Antibiotic-free selection in E. coli: new considerations for optimal design and improved production

Culture time

Plasmid without cer

With cer orientation1

With cer orientation2

1 h

87%

100%

100%

2 h

IPTG addition

3 h

67%

100%

100%

5 h

1%

50%

34%

25 h

0%

9%

8%

  1. The contribution of the cer fragment in increasing the plasmid maintenance over time is again clearly established with a different antigen, the difference observed according to the relative orientation of cer is in the opposite direction as compared to AlpA and likely to be non-significative.
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Microbial Cell Factories

ISSN: 1475-2859

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