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Table 1 Example of Helicobacter pylori AlpA protein produced in erlen flask in absence of Kanamycin

From: Antibiotic-free selection in E. coli: new considerations for optimal design and improved production

Culture time

Plasmid without cer

With cer orientation1

With cer orientation2

2 h(IPTG added)

97%

100%

100%

4 h

25%

100%

100%

6 h

20%

75%

100%

23 h

3%

79%

97%

  1. The contribution of the cer fragment in increasing the plasmid maintenance over time is clearly established, a slight difference is observed according to the relative orientation of cer but should be confirmed by additional experiments