From: Antibiotic-free selection in E. coli: new considerations for optimal design and improved production
Culture time | Plasmid without cer | With cer orientation1 | With cer orientation2 |
---|---|---|---|
2 h(IPTG added) | 97% | 100% | 100% |
4 h | 25% | 100% | 100% |
6 h | 20% | 75% | 100% |
23 h | 3% | 79% | 97% |