Figure 4From: Antibiotic-free selection in E. coli: new considerations for optimal design and improved productionHomologous recombination process allowing assembly of a functional ccdA encoding gene. After transformation of SE1 bacteria expressing the ccdB gene product with linearized plasmid DNA, a functional ccdA gene is assembled through homologous recombination. The expression of the ccdA gene product allows survival of the recipient strain and provides a positive selection ensuring the elimination of the Kanamycine resitance gene.Back to article page