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Figure 3 | Microbial Cell Factories

Figure 3

From: Antibiotic-free selection in E. coli: new considerations for optimal design and improved production

Figure 3

Head to head comparison of the expression of the same antigen with 3 different vectors. Helicobacter pylori AlpA protein was produced in erlen flask in absence of Kanamycin using three differents vectors: pM.H.P3.1 (control vector -cer), pSP3 (antibiotic-free vector -cer) and pSP5 (antibiotic-free vector +cer). Total lysates of induced and non induced bacteria were loaded on SDS-PAGE and a densitometry quantification of the recombinant protein was performed using GeneTools software. This quantification is expressed as a percentage of the protein of interest compared to total proteins. The results show the contribution of both antibiotic-free system and cer locus in protein expression improvement (antibio-free + cer > antibio-free - cer > standard - cer).

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