Figure 2
From: Antibiotic-free selection in E. coli: new considerations for optimal design and improved production
Follow-up of Plasmid stability and Optical density for cultures in fermenter (1 litre scale) upon induction at early stage of growth. As early as 2 hours after induction the % of P+ cells start decreasing and drops dramatically after 4 hours for the standard system. Virtually no decreasing effect is observed with the antibiotic-free selection in the same conditions.