Side effects of chaperone gene co-expression in recombinant protein production

Table 1 Main undesired side effects observed during chaperone co-production on the quality and yield of recombinant proteins produced in E. coli, as exemplified by representative studies.

Chaperone/Chaperone set	Recombinant protein	Effects on protein production	References
DnaKJE	Horseradish peroxidase	Growth inhibition	[31]
DnaKJ	Aggregation-prone GFP	Proteolyis, reduced yield and lower conformational quality	[20, 21]
DnaKJE and/or Trigger Factor	Guinea pig liver transglutaminase	Reduced specific activity	[74]
DnaKJE, ClpB and GroELS	Basic fibroblast growth factor	Reduced yield	[22]
DnaKJE-GroELS-ClpB and Trigger Factor	Human protein kinase catalytic domains	Increased soluble aggregate formation	[43]
GroELS	Basic fibroblast growth factor	Proteolysis, reduced yield	[22]
Trigger Factor and GroELS	N-acyl-D-amino acid amidohydrolases	Reduced specific activity	[75]
GroELS	Fab Antibody Fragment	Reduced yield	[46]
GroELS	scFv antibody fragment	Reduced solubility	[76]
GroELS	Cyclodextrin glycosyltransferase	Reduced specific activity	[32]

ISSN: 1475-2859