Protein solubility and differential proteomic profiling of recombinant Escherichia coli overexpressing double-tagged fusion proteins

Cheng, Chung-Hsien; Lee, Wen-Chien

doi:10.1186/1475-2859-9-63

Table 1 List of identified proteins with altered expression after induction.

From: Protein solubility and differential proteomic profiling of recombinant Escherichia coli overexpressing double-tagged fusion proteins

Spot No.	Gene name	NCBI or SwissProt accession no.	Description of protein	Classification^a	Theoretical		Fold change^b
					M.W. (Da)	pI	BL3/BL0	2TK3/2TK0	NA3/NA0	EP3/EP0
1	clpB	gi\|15832709	Protein disaggregation chaperone	H	95697	5.37	1.01	1.32	1.63	2.19*
2	groEL	gi\|15804735	Chaperonin GroEL	H	57447	4.81	1.24	1.70*	1.23	1.73*
3	hslU	gi\|170769640	Heat shock protein HslVU, ATPase subunit HslU	H	49678	5.24	0.96	2.20	0.70	3.5*
4	ibpA	gi\|15804286	Heat shock protein IbpA	H	15764	5.77	n.d.^c	∞^d	∞	∞
5	ibpB	gi\|30064996	Heat shock protein IbpB	H	16083	5.19	n.d.	∞	∞	∞
6	acnB	gi\|170683451	Aconitate hydratase 2	C	91078	5.22	1.56	0.70	1.21	1.5*
7	aceE	gi\|15799798	Pyruvate dehydrogenase subunit E1	C	99948	5.46	0.19*	0.67	0.13*	0.59
8	atpH	gi\|145398	ATPase delta-subunit	C	19420	4.93	0.61	0.65	0.63*	0.56*
9	maeB	gi\|222157177	NADP-dependent malic enzyme	C	82886	5.34	1.48	3.18*	2.82	2.20*
10	mdh	gi\|440049	Malate dehydrogenase	C	29527	5.73	2.08*	1.37	1.39*	1.11
11	pckA	gi\|16131280	Phosphoenolpyruvate carboxykinase	C	59891	5.46	1.28	1.14	1.17	1.34*
12	sdhA	gi\|193065522	Succinate dehydrogenase, flavoprotein subunit	C	65024	5.85	1.02	2.89*	2.15*	1.90
13	adk	gi\|15800203	Adenylate kinase	N	23628	5.55	0.84	0.77	0.69*	0.77*
14	apt	gi\|16128453	Adenine phosphoribosyltransferase	N	19847	5.26	0.66*	0.53*	0.59*	0.62*
15	dcd	gi\|15802547	Deoxycytidine triphosphate deaminase	N	21352	5.62	1.15	0.45	6.00*	7.83*
16	deoB	gi\|91214099	Phosphopentomutase	N	44707	5.15	0.36	0.25*	0.25*	0.25*
17	dut	gi\|16131511	Deoxyuridinetriphosphatase	N	16202	5.03	0.87	0.63*	0.42*	0.38*
18	prsA	gi\|15801436	Ribose-phosphate pyrophosphokinase	N	34401	5.23	0.61*	0.49*	0.71*	0.54*
19	purH	gi\|26250778	Bifunctional phosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase	N	57656	5.66	1.00	0.86	2.47*	1.43
20	melA	gi\|15804711	Alpha-galactosidase	CH	51219	5.58	7.58*	1.80*	2.21*	1.74*
21	gmhA	gi\|26106668	Phosphoheptose isomerase	CH	27392	8.24	0.96	2.09*	0.32*	0.43*
22	lacA	gi\|15800071	Galactoside O-acetyltransferase	CH	22965	6.38	20.37	∞	∞	∞
23	rbsB	gi\|15804351	D-ribose transporter subunit RbsB	CH	30919	6.85	3.20*	3.01	2.69*	0.70
24	rpiA	gi\|15803449	Ribose-5-phosphate isomerase A	P	22903	5.20	1.61*	0.54*	0.64*	0.54*
25	tktA	gi\|193063507	Transketolase	P	72464	5.43	2.88	n.d.	0.30*	n.d.
26	dppA	gi\|193068548	Dipeptide ABC transporter, periplasmic dipeptide-binding protein	X	60467	6.21	2.75*	1.25	1.83	1.84*
27	oppA	gi\|15801469	Oligopeptide transport; periplasmic binding protein	X	61099	5.95	2.58*	2.41*	1.96*	1.40*
28	tnaA	gi\|15804305	Tryptophanase	X	53791	5.88	6.40*	2.65*	1.77*	1.39
29	gcvT	gi\|16130807	Aminomethyltransferase, tetrahydrofolate-dependent, subunit (T protein) of glycine cleavage complex	X	40235	5.36	1.93*	1.05	0.90	1.40*
30	glpK	gi\|15804515	Glycerol kinase	X	57226	5.36	0.24	0.34*	0.26*	0.77
31	gatD	gi\|91211377	Galactitol-1-phosphate dehydrogenase	X	37874	5.94	1.85	1.46	1.98*	0.75
32	garR	gi\|89109891	Tartronate semialdehyde reductase	X	30754	5.58	n.d.	n.d.	1.94*	n.d.
33	ackA	gi\|1359437	Acetate kinase	X	43488	5.76	0.53	0.55*	0.64*	0.55*
34	panC	gi\|16128126	Pantothenate synthetase	X	31692	5.91	1.57	0.73	1.87	3.13*
35	luxS	gi\|15803206	S-ribosylhomocysteinase	X	19603	5.18	1.84	0.70*	0.53*	0.45*
36	hdhA	gi\|15802033	7-alpha-hydroxysteroid dehydrogenase	X	26990	5.22	1.19	0.40*	0.61*	0.59*
37	iscU	gi\|15803056	Scaffold protein	X	14011	4.82	0.70	0.68	0.36*	0.31*
38	minE	gi\|15801396	Cell division topological specificity factor MinE	X	10286	5.15	2.00*	0.82	0.47*	0.88
39	uspA	gi\|15804030	Universal stress protein	X	16113	5.11	3.60*	1.10	0.42*	0.78
40	ilvC	B1X9Z0	Ketol-acid reductoisomerase	X	54376	5.20	1.12	0.65	0.37*	0.74
41	ybl119	gi\|242378351	Periplasmic binding protein and sugar binding domain, lacI family	X	33786	5.68	2.19	∞	∞	2.48
42	yceI	gi\|218704465	Polyprenyl-pyrophosphate binding protein	X	20886	5.56	1.82	0.54*	0.40*	0.47*
43	ydhR	gi\|170680041	YdhR protein	X	11297	5.09	1.10	0.57*	0.43*	0.63*
44	ampC	gi\|41817	Beta lactamase TEM6	R	31646	5.93	n.d.	11.28*	5.41*	7.12*
45	ampC	gi\|41817	Beta lactamase TEM6	R	31646	5.93	n.d.	∞	∞	∞
46	ampC	gi\|41817	Beta lactamase TEM6	R	31646	5.93	n.d.	∞	∞	∞
47	ampC	gi\|41817	Beta lactamase TEM6	R	31646	5.93	n.d.	∞	∞	∞
48	ampC	gi\|41817	Beta lactamase TEM6	R	31646	5.93	n.d.	8.09*	42.78*	51.26*
49	ampC	gi\|41817	Beta lactamase TEM6	R	31646	5.93	n.d.	3.83	7.05*	6.02*

^aClassification: H, chaperone & heat shock protein; C, central metabolism and TCA cycle; N, nucleotide and nucleoside metabolic process; CH, carbohydrate metabolic and catabolic processes; P, pentose phosphate pathway; X, other function or pathway; R, antibiotic resistance.
^bRelative %volume of 3-h sample/0-h sample in each group. BL, E. coli BL21; 2TK, E. coli BL21 harboring pGEX-2TK; NA, E. coli BL21 harboring pGEX-2TK-nanA-5R; EP, E. coli BL21 harboring pGEX-2TK-2ep-5D. The fold change was calculated by dividing the value of 3-h sample by the value of 0-h sample. If this number was less than one, the fold change is the reciprocal of this number (e.g. 0.70 is reported as 1.43 fold down-regulation).
^cn.d., not detected.
^d∞: present only in the 3-h sample.
* denotes significant differential expression.

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