Figure 5

Western blot analysis of sigma 32 in recombinant protein expressing strains. Western analyses were performed for sigma 32 expressions in GST-, GST-GlcNAc 2-epimerase-5D-, and GST-Neu5Ac aldolase-5R-expressing E. coli BL 21 induced for 0, 1, 2, and 3 h. The samples marked with C (in upper panel) referre to the protein mixture loaded in each gel to serve as the inter-membrane control. In the graphical plot, the arbitrary unit was calculated by dividing the image intensity of the anti-σ³² band on developed film by that of the identical control on the same film. Error bars stand for S.D. (n = 3).