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Figure 3 | Microbial Cell Factories

Figure 3

From: Protein solubility and differential proteomic profiling of recombinant Escherichia coli overexpressing double-tagged fusion proteins

Figure 3

Assay of expressed proteins by SDS-PAGE and ELISA. SDS-PAGE of protein extracts from E. coli BL21 harboring pGEX-2TK-nanA-5R after 3 h of IPTG induction (A), pGEX-2TK-2ep-5D (B) and pGEX-2TK (C). Quantification of GST-fused proteins in each extraction was done by ELISA using an anti-GST antibody (D). The molecular weights of GST-Neu5Ac aldolase-5R, GST-GlcNAc 2-epimerase-5D, and GST are 59, 70 and 28 kDa, respectively, and the positions are indicated by arrows. Lane M: protein marker; lanes 1-3: the first, second and third extractions from cell pellets; lane P: extraction from aggregates as described in the Methods section. Soluble percentage was defined as the percentage of total soluble protein in extractions 1, 2 and 3 as a function of the sum of total soluble protein and insoluble protein (extraction P). Error bars stand for S.D.

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Microbial Cell Factories

ISSN: 1475-2859

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